<p>(a) Total BM cells harvested for flow cytometry were not different between HF and RD animals at any time point, but a trend of increase was observed at 6W. (b) The total number of B-cells was not changed at 2D or 1W contrary to a decline in the phenotypic population at 1W. However, B-cells were reduced at 6W, despite a slight increase in total cellularity, demonstrating the degree of population reduction. (c,d) Total population sizes of both T-cells and Myeloid lineages tracked well with the phenotypic population proportions. *<i>P</i><0.05 vs. RD.</p
<p>Wild-type C57Bl/6 mice were placed on a SCD or HFD for 26 weeks (Panels A and B), 3-weeks (Panel ...
Purpose Obesity is a chronic, inflammatory state. Blood monocyte concentrations/proportions have be...
<p><b>A)</b> In the first panel, FACS plots of splenic cells plotted against CD19 and B220 in the di...
<p>(a) A two-color flow cytometric method was used to isolate leukocyte populations of the lymphocyt...
The bone marrow (BM) niche is the primary site of hematopoiesis, and cues from this microenvironment...
The bone marrow (BM) niche is the primary site of hematopoiesis, and cues from this microenvironment...
<p>(a) A representative gating scheme to isolate KLS and SP-KLS cells is shown. HSCs (SP-KLS) were a...
<p>(a,b,d) The percentage of BM space taken up by adipose tissue, as assessed by histology, increase...
Growth rate and competition effects are similar to the impact on HSCs. Lower competition leads to gr...
<p>Peripheral blood leukocyte counts were determined after 8 weeks of high-fat diet (n = 8). Leukocy...
<p><b>A)</b> The first panel represents the FACS plot of the total CD3<sup>+</sup>NK1.1<sup>−</sup> ...
<p><b>A)</b> The first panel of FACS plots represents the plots of CD19 and B220 from the three diet...
<p>Wild-type C57BL/6 mice were fed either normal diet or high-fat diet for 12 or 16 weeks. Kupffer c...
a<p>Following treatment with RU486 (R), RU486 plus leptin (RL), leptin (L), vehicle respectively, mi...
<p>(A) Myeloid cells (CD11b<sup>+</sup>) are diminished in the bone marrow in the absence of FN in o...
<p>Wild-type C57Bl/6 mice were placed on a SCD or HFD for 26 weeks (Panels A and B), 3-weeks (Panel ...
Purpose Obesity is a chronic, inflammatory state. Blood monocyte concentrations/proportions have be...
<p><b>A)</b> In the first panel, FACS plots of splenic cells plotted against CD19 and B220 in the di...
<p>(a) A two-color flow cytometric method was used to isolate leukocyte populations of the lymphocyt...
The bone marrow (BM) niche is the primary site of hematopoiesis, and cues from this microenvironment...
The bone marrow (BM) niche is the primary site of hematopoiesis, and cues from this microenvironment...
<p>(a) A representative gating scheme to isolate KLS and SP-KLS cells is shown. HSCs (SP-KLS) were a...
<p>(a,b,d) The percentage of BM space taken up by adipose tissue, as assessed by histology, increase...
Growth rate and competition effects are similar to the impact on HSCs. Lower competition leads to gr...
<p>Peripheral blood leukocyte counts were determined after 8 weeks of high-fat diet (n = 8). Leukocy...
<p><b>A)</b> The first panel represents the FACS plot of the total CD3<sup>+</sup>NK1.1<sup>−</sup> ...
<p><b>A)</b> The first panel of FACS plots represents the plots of CD19 and B220 from the three diet...
<p>Wild-type C57BL/6 mice were fed either normal diet or high-fat diet for 12 or 16 weeks. Kupffer c...
a<p>Following treatment with RU486 (R), RU486 plus leptin (RL), leptin (L), vehicle respectively, mi...
<p>(A) Myeloid cells (CD11b<sup>+</sup>) are diminished in the bone marrow in the absence of FN in o...
<p>Wild-type C57Bl/6 mice were placed on a SCD or HFD for 26 weeks (Panels A and B), 3-weeks (Panel ...
Purpose Obesity is a chronic, inflammatory state. Blood monocyte concentrations/proportions have be...
<p><b>A)</b> In the first panel, FACS plots of splenic cells plotted against CD19 and B220 in the di...