The neuroprotective effect of GL against cerebral I/R injury in rats.

<p>A) Cerebral infarction induced by MCA occlusion was evaluated 48 h after reperfusion by TTC staining of brain slices from rats treated with different doses of GL or NS. Sham-operated rats showed no infarction or TTC staining. The infarct volumes were quantified using computerised image analysis. B) Neurological scoring was carried out according to the categories described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0089450#s2" target="_blank">Materials and Methods</a>. C) Neurological deficits in rats after MCA occlusion were examined using the rota-rod test. In the rota-rod test, trials were performed at 3 different speeds, and the time intervals running on the rod were determined for each rat after reperfusion. Values are means±SEM, n = 8 for each group. *P<0.05, **P<0.01 (t test).