Secreted Gluc permeates from the established tissue cage infection loci to the blood stream and serves as an <i>ex situ</i> indicator for the localized tissue cage infection.

<p>Mouse plasma was collected at the denoted time points during tissue cage infection and measured for Gluc activity present in plasma (details seen Experimental Procedures). (A) Mouse tissue cages were inoculated with 10³ CFU of ML6189 or ATCC25922 at the beginning of infection. (B) Mouse tissue cages were inoculated with 10⁵ CFU of ML6189 or ATCC25922 at the beginning of infection. Unpaired and two tailed Student's <i>t</i>-test was used for all two comparisons with one variable (indicated by the connected lines in the figure), <i>P</i><0.01 was considered as statistically significant and marked by “*”. For all panels, the mean value for each group of samples is represented by a black bar. Data from two independent experiments were pooled.