Effect of rapamycin on FL-gB expression.

<p>(<b>A</b> left panel) High Five cells were infected with bBst2x at CCI 2.5 with m.o.i. 5 in the presence of either 0.1% DMSO (cnt) or the indicated final concentrations of rapamycin, harvested at 72 h.p.i. and relative FL-gB expression analysed by immunoblot with ECL mean signals of control samples set as 1; (right panel) anti-gB TM immunoblot on detergent-soluble protein extracts from High Five cells infected as above in the presence of 0.1% DMSO (cnt) or with 50 nM rapamycin added at the indicated time points. (<b>B</b> left panel) High Five cells were infected as in (A) in the presence of 50 nM rapamycin (diamonds) or DMSO alone (stars) and cell cultures were sampled at the indicated h.p.i. to measure viral titers (solid lines) and cell volume (dashed lines); (right panel) High Five cell were infected as in (A right panel) and the recorded cell volume plotted as cell swelling inhibition (stars) along with relative FL-gB expression (diamonds) detected in the same culture samples. (<b>C</b>) Kinetic expression of FL-gB and <i>Ac</i>MNPV gp64 analysed by immunoblot of detergent-soluble protein extracts from High Five cell cultures infected and sampled as in (B left panel).