BEVS-driven FL-gB expression screening.

<p>(<b>A</b>) bBst2x was used to infect Sf9 (left panel) or High Five (right panel) cells at 0.3 (circles), 1 (diamonds), 2.5 (triangles) or 4 (crosses) ×10⁶ cell/mL (CCI)) with mutliplicities of infection (m.o.i.) of 0.1, 1, 3, 5 or 10 pfu/cell. Cells were harvested at 72 hours after infection and detergent-soluble protein extracts were analysed by densitometric analysis of anti-gB TM immunoblots. Overall relative quantification was made by comparing samples from different experimental series in the same immunoblots (hereafter n = 3 with error bars representing 95% confidence interval, C.I.) and setting as 100% the mean of most intense signals. (<b>B</b>) Comparison of FL-gB expression pattern and kinetics in Sf9 and High Five cell lines, bBst2x-infected with m.o.i. 5 at CCI 0.3 (Sf9) or 2.5 (High Five, Hi5). Cells were sampled at the indicated hours <i>post</i>-infection (h.p.i.) and detergent-soluble cell extracts probed in immunoblot (IB) with anti-gB TM. Positions of the molecular weight markers are indicated and expressed in kDa. (<b>C</b>) Theroretical FL-gB relative volumetric yields computed from (A) for m.o.i. 5 series, according to CCI and cell viability at 72 h.p.i.