Phosphoinositide phosphorylation links PKG to gliding in <i>P. berghei</i> ookinetes.

<p>(A) Ookinete gliding speed of PI4K^S534A and PI4K^S534A/S538A ookinete mutants. Values are representative of 20 individual ookinetes from two independent biological replicates. (B) Ratio of peripheral to cytosolic fluorescence intensity from optical sections taken of different ookinetes from the experiment shown in (C); <i>n</i> = 10 sections from different ookinetes. Statistical analysis was carried out using a two-tailed <i>t</i> test. (C) Confocal immunofluorescence images of fixed ookinetes showing the effect of 0.5 µM C2 on the cellular distribution of a C-terminally HA tagged PIP5K (PBANKA_020310) expressed from its endogenous promoter. Scale bar, 5 µm. (D) Relative quantification of PI, PIP, PIP₂, and PIP₃ levels after 10 min treatment with 0.5 µM C2 or DMSO in PKG-HA ookinetes. (E) As in (D) but for PKG^T619Q-HA ookinetes. Error bars in (D) and (E) show standard deviations of two biological replicates. The <i>p</i> values are from two-tailed <i>t</i> test. See also <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001806#pbio.1001806.s005" target="_blank">Figure S5</a>.