Effects of down regulation of p62/SQSTM1 in SCC35 cells and association of p62/SQSTM1protein level with drug resistance in both SCCHN and breast cancer cells.

<p><b>A</b>) Western blotting shows knockdown of p62/SQSTM1 in SCC35-p62KD cell line compared to SCC35-control (left). The p62/SQSTM1 bands were quantified and shown graphically on the right. <b>B</b>) Dose-response curves of SCC35-p62KD, SCC35-control, parental cells (SCC35), and SCC61 cells to MK-2206. <b>C</b>) Detection of p62/SQSTM1 protein expression in panels of SCCHN (Top) and breast cancer cell lines (Middle) by western blotting as described in Methods. Dose-responsive curves of the panel of breast cancer cell lines treated with MK-2206 (Bottom). <b>D and E</b>) Reduced expression of Nrf2 (<b>D</b>) and NQO1 (<b>E</b>) in SCC35-p62KD and SCC35-control cells. Western blotting shows duplicate samples from each derived cell line. Graph represents quantified fold change normalized to control.