Diagrammatic representation of the DNA constructs, including control plasmids, used for immunization.

<p>The construct for <i>P. yoelii</i> used mouse MIP3α, while the <i>P. falciparum</i> construct employed the sequence for human MIP3α. M refers to MIP3α. CSP refers to a segment of the <i>Plasmodium yoelii</i> circumsporozoite protein; and MCSP refers to the fusion protein of MIP-3α and a segment of the <i>P</i>. <i>yoelii</i> circumsporozoite protein. Also shown are the positions of the leader sequence, spacer, and the myc tag. The leader sequence for the <i>P. yoelii</i> construct is the interferon–γ induced protein 10 secretion leader sequence; the leader sequence for the <i>P. falciparum</i> construct is the human tissue plasminogen activator leader sequence <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0090413#pone.0090413-Pennica1" target="_blank">[46]</a>. The MCSP and hMfCSP nucleotide sequences are provided in the supplementary materials.