Suppression of IL-17 and IL-22 by GAG is dependent on IL-1Ra.

<p>(A) IL-17, IL-22 and IFN-γ concentrations in culture supernatants of PBMCs stimulated for 7 days with heat inactivated <i>A. fumigatus</i> conidia 1×10⁷/ml, IL-1β/IL-23 (50/100 ng/ml) or IL-12/IL-18 (50/100 ng/ml) in the presence or absence of recombinant human IL-1Ra (10 ng/ml). Data are represented as mean +/− SEM. (B) Inhibition of IL-1β/IL-23 (50/100 ng/ml) induced IL-17 and IL-22 by GAG (10 µg/ml) in human PBMCs in the presence of isotype control (10 µg/ml) or anti-IL-1Ra (10 µg/ml). The IL-17 and IL-22 production by IL-1β/IL-23 in absence of GAG was set at 100% and mean percentage changes relative to the control are represented +/− SEM.