Similarity between the secondary and tertiary structural features of holo-PfRd, Apo-1 PfRd and Apo-2 PfRd.

<p><i>Panel a</i>: Far-UV CD spectra of Apo-1 (blue), Apo-2 (red) and holo-PfRd (black). The spectrum has two bands. The 203 nm band owes to contributions from polyproline type II (PP-II) and random coil structures. The 225 nm band owes to contributions from secondary structures and aromatic residues. <i>Panel b</i>: Near-UV CD spectra of Apo-1 (blue), Apo-2 (red) and holo-PfRd (black). All bands owe to aromatic contributions. <i>Panel c</i>: Fluorescence emission spectra of Apo-1 (blue), Apo-2 (red) and holo-PfRd (black). The emission maximum of Apo-1 PfRd can be seen to be red-shifted by 2–3 nm.