The GC-ACS is required for GC-ARS function.

<p>Wild type (WT) and mutant (MUT) alleles of the twelve ARSs indicated were cloned into a <i>URA3</i> ARS-less vector and used to transform <i>ura3</i> yeast on selective medium plates lacking uracil. Plates were grown at 30°C for five days before pictures were taken. Colony formation indicates plasmid maintenance and ARS activity. The GC-ACS was positioned <15 bp away from the 5′ endpoint in all ARS sequences. The sequences of the fragments tested are listed in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004169#pgen.1004169.s012" target="_blank">Table S4</a>.