Endogenous expression of ABCG2 and SP fractions in CML patients.

<p>(<b>a</b>) The ABCG2 transcript in CML patients was determined by real-time RT-PCR using the RQ values with <i>GAPDH</i> mRNA as the endogenous control. In contrast to normal donors, no substantial difference in the ABCG2 transcript levels was detected in CML patients. *<i>P</i><0.05. (<b>b</b>) ABCG2+ cells were labeled by CD338-PE and detected by FACS, using IgG2b-PE as the isotype control. There were no significant differences among the groups. *<i>P</i><0.05. (c) Significantly higher percentages of SP cells were observed in CML blasts at AP/BP using flow cytometry. *<i>P</i><0.05.