Temporal effect of FGF9 on the expression levels of phospho-Akt, -JNK, -p38, and -ERK1/2 in MA-10 cells.

<p>MA-10 cells were treated without or with FGF9 (50 ng/ml) for the indicated times. The expression levels of phospho- Akt (A), -JNK (B), -p38 (C), and -ERK1/2 (D) were detected by immunoblotting. Immunoblots represent the observations from one single experiment repeated at least in three different times. The integrated optical density of each protein was normalized to β-actin (43 kDa) in each lane. The fold difference of each of phospho-Akt, -JNK, -p38, and -ERK1/2 in the treatment group was normalized to the control group at each time point, and the mean ± SEM of three separate experiments is shown. Different letters above the bars indicate statistically significant differences between treatments (<i>p</i><0.05). P represents the positive control.