<i>In vitro</i> culture with IL-7 increases IFN-α-induced activation of STAT1, STAT2 and STAT3 in CD4 but not CD8 T cells.

<p>Isolated CD4 (<b>a</b>) and CD8 (<b>b</b>) T cells from a healthy donor were cultured and treated as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003976#ppat-1003976-g002" target="_blank">Figure 2</a>. Cell lysates were analyzed by Western blotting with antibodies specific to p-STAT1, t-STAT1, p-STAT2, t-STAT2, p-STAT3, t-STAT3, p-STAT5 and t-STAT5. An antibody to actin was used to confirm even protein loading. Numbers represent the ratio of the densitometry values of band densities on western blots calculated using the values of the unstimulated cells cultured for 3 days with media only as the baselines. <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003976#s2" target="_blank">Results</a> are representative of at least 3 different donors.