Chemokine production and chemotactic activity of homogenates of skin wound.

<p><i>A-C</i>, chemokine production of skin wound at 72 h (n = 15). WT and Fpr-deficient mice were subjected to full-thickness skin wound and the wounds were harvested at 72 h after injury and then homogenized for chemokine measurement with ELISA. <i>D</i>, homogenate-induced migration of parental and Fpr-transfected HEK293 cells. Migrating cells in response to different concentration of the homogenate in 3 HPF were counted. *, significantly increased migrating cells in response to homogenates as compared to medium control (0) (<i>p</i><0.05). <i>E</i>, chemotactic activity of skin homogenates for neutrophils from WT and Fpr-deficient mice. *, significantly increased migrating neutrophils in response to homogenates as compared to medium control (0) (<i>p</i><0.05).