ODR-8 acts in an early step in ODR-10 trafficking to the AWA cilia.

<p>(A) Localization of ODR-10-GFP in AWA neurons of <i>odr-8(ky31)</i>, <i>unc-101(m1)</i> and <i>odr-8(ky31); unc-101(m1)</i> mutants. Thresholded images were obtained as described in the methods. (B) Quantitative analysis of ODR-10 and TRAM-1 co-localization in AWA neurons of <i>odr-8(ky31)</i>, <i>unc-101(m1)</i> and <i>odr-8(ky31); unc-101(m1)</i> mutants. (C). Localization of ODR-10-GFP in AWA neurons in <i>rab-2</i> mutants. Arrowheads indicate the AWA cilia, white arrows point to the AWA cell body and red arrows show the AWA processes in the nerve ring. In <i>rab-2(n777)</i> mutants, 23 out of 29 animals displayed normal ODR-10-GFP localization in AWA cilia, and 6 out of 29 show the ODR-10-GFP localization defects in AWA cilia. (D–E). Quantification of ODR-10-GFP signals in the cilia (D) and axons (including cell body) (E) of AWA neurons. **, <i>p</i><0.01 (ANOVA, Bonferroni's multiple comparisons test); for E, <i>rab-2(n777)</i> shows <i>p</i><0.01 compared to all other genotypes plotted.