<p>Electrophoretic profiles and antibody labelling of PrP<sup>res</sup> as detected by mAbs Sha31 (A) and 12B2 (B) in brain extracts from BoPrP-Tg110 mice inoculated with the different BSE-derived prions: cattle-BSE (lane 1), sheep-BSE (lane 2), OvTg-BSE (lane 3), pig-BSE (lane 4), PoTg-BSE (lane 5), mouse-BSE (lane 6), MoTga20-BSE (lane 7), human-vCJD (lane 8), HuTg-BSE (lane 9). Brain extracts from BoPrP-Tg110 mice inoculated with atypical cattle-BSE H (lane 10), sheep-scrapie (lane 11) and mouse-RML (lane 12) were included as a control non-BSE related prion propagated in the same mouse model. Panels A and B were loaded with the same quantities of PrP<sup>res</sup> extracted from each sample. MW, molecular weight in kilodaltons.</p
<p>Mice were challenged by oral (lane 5–8) or the intra-cerebral route (lanes 1–4). A) Western blot ...
AbstractTransgenic (Tg) mice carrying four extra octapeptide repeats (OR) in the bovine PrP gene (10...
<p>Except for one undigested lane (−), brain samples were digested with PK (+ in panel A). All inocu...
<p>Electrophoretic profiles and antibody labelling of PrP<sup>res</sup> as detected by mAbs Sha31 (A...
<p>PrP<sup>res</sup> was detected by western blot using the mAbs Sha31 (A) and 12B2 (B) in the diffe...
<p>PMCA reactions were seeded with BSE brain material (10<sup>−2</sup> dilution) from different spec...
Transgenic mouse lines expressing different levels of the bovine prion protein gene (boPrP(C)) were ...
<p>Immunoblots of brain PrP<sup>res</sup> detected with SHA 31 and 12B2 mAbs. Similar quantities of ...
Ctrl; saline. dpi; days post-inoculation. *Numbers indicate the first (1) and second (2) passages. I...
<p>PrP<sup>res</sup> was detected with the Sha31 monoclonal antibody in the different BSE-derived pr...
<div><p>(A) Primary or secondary (lane 6) transmission to tgBov mice. BSE-type inocula include cattl...
<p>Immunoblots of brain PrP<sup>res</sup> detected with SHA 31 Mab. Similar quantities of PrP<sup>re...
<p>Immunoblots of brain PrP<sup>res</sup> detected with SHA 31 Mab. Similar amount of brain tissue e...
<p>All mice were inoculated with the same vCJD prion isolate. Immunoblots were analysed by enhanced ...
<p>A) BSE-derived prions propagated in several host species (cattle-BSE, sheep-BSE, pig-BSE, mouse-B...
<p>Mice were challenged by oral (lane 5–8) or the intra-cerebral route (lanes 1–4). A) Western blot ...
AbstractTransgenic (Tg) mice carrying four extra octapeptide repeats (OR) in the bovine PrP gene (10...
<p>Except for one undigested lane (−), brain samples were digested with PK (+ in panel A). All inocu...
<p>Electrophoretic profiles and antibody labelling of PrP<sup>res</sup> as detected by mAbs Sha31 (A...
<p>PrP<sup>res</sup> was detected by western blot using the mAbs Sha31 (A) and 12B2 (B) in the diffe...
<p>PMCA reactions were seeded with BSE brain material (10<sup>−2</sup> dilution) from different spec...
Transgenic mouse lines expressing different levels of the bovine prion protein gene (boPrP(C)) were ...
<p>Immunoblots of brain PrP<sup>res</sup> detected with SHA 31 and 12B2 mAbs. Similar quantities of ...
Ctrl; saline. dpi; days post-inoculation. *Numbers indicate the first (1) and second (2) passages. I...
<p>PrP<sup>res</sup> was detected with the Sha31 monoclonal antibody in the different BSE-derived pr...
<div><p>(A) Primary or secondary (lane 6) transmission to tgBov mice. BSE-type inocula include cattl...
<p>Immunoblots of brain PrP<sup>res</sup> detected with SHA 31 Mab. Similar quantities of PrP<sup>re...
<p>Immunoblots of brain PrP<sup>res</sup> detected with SHA 31 Mab. Similar amount of brain tissue e...
<p>All mice were inoculated with the same vCJD prion isolate. Immunoblots were analysed by enhanced ...
<p>A) BSE-derived prions propagated in several host species (cattle-BSE, sheep-BSE, pig-BSE, mouse-B...
<p>Mice were challenged by oral (lane 5–8) or the intra-cerebral route (lanes 1–4). A) Western blot ...
AbstractTransgenic (Tg) mice carrying four extra octapeptide repeats (OR) in the bovine PrP gene (10...
<p>Except for one undigested lane (−), brain samples were digested with PK (+ in panel A). All inocu...