RTA expression alters vFLIP expression.

<p>A. vFLIP protein is decreased in the presence of RTA. 293T cells were transfected with myc-vFLIP, RTA, or empty vector where indicated. 48 hrs post-transfection, cells were harvested and analyzed by SDS-PAGE followed by western blot with antibodies against myc(vFLIP), RTA, and tubulin. B. RTA induced degradation of vFLIP is dose dependent. 293T cells were transfected with myc-vFLIP and increasing levels of RTA or empty vector control where indicated. 48 hrs post transfection, cells were harvested and analyzed by SDS-PAGE followed by western blot with antibodies against myc(vFLIP), RTA, and tubulin. C. RTA induces proteasomal degradation of vFLIP. 293T cells were transfected with myc-vFLIP and RTA or empty vector control where indicated. The proteasome inhibitor MG132 was added 12 hrs post transfection at the indicated concentrations. DMSO was added in the absence of MG132 in lanes 1 and 2. 48 hrs post transfection, cells were harvested and analyzed by SDS-PAGE followed by western blot with antibodies against myc(vFLIP), RTA, and tubulin.