Increased Levels of Extracellular and Intracellular p24 Gag and gp120 Env Proteins by Nedd4.

<p>293 cells were transiently transfected with a plasmid DNA expressing a clade B Mosaic Gag (A) or HIV-1_BaL gp120 (B) and increasing concentrations of hNedd4 as described (Materials and Methods). At 48 hr post-transfection, cell supernatants or cell lysates were assayed for p24 (A) or gp120 (B) by ELISA. Levels (pg/ml) of p24 (A) and gp120 (B) are represented graphically for supernatants (blue line) or cell lysates (red line).