Histological features of rejected non-immunosuppressed alloveins.

<p>Representative light microscopic photograph showing the histological features of alloveins in non-immunosuppressed rats (group B) 30 days following transplantation into the infrarenal abdominal aorta: A - vein stained for immunoglobulins with a fluorescein isothiocyanate-conjugated antibody (Chemicon International Inc, Temecula, California, USA). No free clusters of immunoglobulins were detected in the allovenous wall. B - vein stained for CD4+ cells with anti-CD4 antibody (OX-8, Cymbus Biotechnology LTD, Southampton, UK) as described previously.<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0091212#pone.0091212-Matia1" target="_blank">[12]</a> Massive infiltration of CD4+ immunocompetent cells (stained brown) led to the destruction of allovenous wall with no histological signs of arterialisation. Original magnification ×100.