Phospho-Akt and Akt expression in post-ischemic brain.

<p>pAkt and pan-Akt levels were analyzed by Western blot (top panels). pAkt expression, normalized to pan-Akt, is shown in the histograms (lower panels). (A) p-Akt levels were reduced in both control and IGF-1-treated groups in the ischemic hemisphere at 4h, as compared to the non-ischemic hemisphere (main effect of ischemia; p<0.05). (B) At 24h, p-Akt expression was significantly increased in the ischemic hemisphere of the IGF-1-treated group as compared to the vehicle-treated group (interaction effect of ischemia and IGF-1). Bars represent mean ± SEM. n = 6 in each group. *: p <0.05.