Superoxide radicals (O₂⁻) detection in the infected macrophages treated with 50 μg/mL KA for 1 h.

<p>(<b>A</b>) Macrophages without infection and untreated. (<b>B</b>) Positive control, interaction with Zymozan (<b>arrows</b>). (<b>C</b>) Macrophage infected with <i>L. (L.) amazonensis</i> (<b>arrows</b>) showed superoxide production inhibition. (<b>D</b>) Macrophages infected with <i>L. amazonensis</i> and treated with KA reverted the inhibitory effect. Bars: 10 μm. (<b>E</b>) Number of infected macrophages that presented formazan deposits. MO+L.a.: macrophages infected with <i>L. amazonensis</i>; MO+L.a.+KA: macrophages infected with <i>L. amazonensis</i> and treated with 50 μg/mL KA; O₂⁺: infected macrophages with formazan deposits; O₂: infected macrophages without formazan deposites. (***p<0.001).