Effects of salusins on the protein expressions of VCAM-1 and MCP-1 in the aorta of mice.

<p>(A) Representative photomicrographs showing immunohistochemical staining for VCAM-1 and MCP-1. Immunoreactivity appears as a result of 3, 3′-diaminobenzidine (DAB) colorimetric reaction. (B) The mean optical densities of VCAM-1 immunostaining. (C) The mean optical densities of MCP-1 immunostaining. (D) Western blotting analysis for VCAM-1 protein expression. (E) Optical density analysis shown as the ratio of VCAM-1 to β-actin. Con: the C57BL/6 control mice; ApoE-/-: the vehicle-treated apoE-/- mice; Sal-α: the salusin-α-treated apoE-/- mice; Sal-β: the salusin-β-treated apoE-/- mice. The results were presented as mean±SD (<i>n</i> = 4-5). **<i>P</i><0.01 compared with the control group, ^#<i>P</i><0.05, ^##<i>P</i><0.01 compared with the apoE-/- group.