Nicardipine suppresses LPS/IFN-γ-induced signaling pathways.

<p>BV-2 microglial cells were pretreated with nicardipine (10 μM) for 60 min, then exposed to LPS (10 ng/ml) plus IFN-γ (10 ng/ml) for another 60 min. Western blot analysis was performed on whole cell lysates, and the signal intensities were normalized to total protein expression. The results are expressed as mean ± S.E.M. from 3 to 4 independent experiments. *, <i>p</i><0.05 compared with the LPS/IFN-γ treatment alone group.