ZEB2 contributes to miR-132- suppressed migration and invasion of NSCLC cells.

<p>(A) The expression of ZEB2 was examined by Western blot in NSCLC cells. (B) The relative mRNA levels of ZEB2 were detected in 45 paired NSCLC primary tumor tissues and their lymph node metastasis counterparts. ZEB2 expression in those tissues was compared by way of Wilcoxon signed-rank test (***<i>P</i><0.001, Student’s t- test). (C) Inverse correlation between miR-132 and ZEB2 expression in NSCLC tissues. ZEB2 expression was analyzed by qRT-PCR and normalized to GAPDH. The miR-132 expression was detected by qRT-PCR analysis and normalized to U6 expression. Statistical analysis was performed using Pearson’s correlation coefficient (r = −0.68, ***<i>P</i><0.001). (D) The cell invasion was detected by Boyden chamber assay in NL9980 or 95C cells transfected with pCMV or pCMV-ZEB2 vectors, respectively. (E, F) The effect of ZEB2 knockdown on the cell migration or invasion was assessed by wound healing or Boyden chamber assay, respectively (**<i>P</i><0.01, Student’s <i>t</i>- test). Additionally, the silencing efficiency of ZEB2 by siRNA was examined by Western blot. (G, H) The wound healing or Boyden chamber assay was used to detect the migration or invasion ability of NL9980 cells with different treatments, respectively (*<i>P</i><0.05, **<i>P</i><0.01, Student’s <i>t</i>- test). Additionally, the silencing efficiency of ZEB2 by siRNA was examined by Western blot.