NLRP1 sequence in inbred and RI rats correlates with rapid macrophage death.

<p>(A) Sequence map of rat NLRP1 variants. This diagram was modified from <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003927#ppat.1003927-Newman1" target="_blank">[4]</a>. Vertical black lines indicate amino acid polymorphisms relative to the protein encoded by allele 1. Approximate NACHT, LRR and CARD domain locations relative to polymorphisms are shown. Macrophage sensitivity to LT-induced pyroptosis for the listed rat strains is from <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003927#ppat.1003927-Newman1" target="_blank">[4]</a> and <i>Toxoplasma</i> sensitivities are from this work. (B–E) Viability measurements for rat BMDMs from LEW, SHR (expressing NLRP1^variant5); CDF, BN or SD (expressing NLRP1^{variant 1,2}); or RI rat strains following infection with <i>Toxoplasma</i> Type I (RH) or Type II (76K or PRU) (MOI 3∶1) by MTT measurements. Data shown are average from three independent experiments with SD (triplicate wells/experiment/condition), except RI strains, which are averages from two experiments (triplicate wells/experiment/condition). Viability values were calculated relative to MTT measurements for uninfected control cells at each time point which were set at 100%. P-values comparing all NLRP1^{variant 1,2} -expressing strains to NLRP1^{variant 5} -expressing strains are <0.001.