The effect of miR-27a/sFRP1 on osteoblast markers in osteogenic hFOBs.

<p>ALP (<b>A</b>), SPP1 and RUNX2 mRNA (<b>B</b>) expression was determined by real-time PCR, and ALP activity (<b>A</b>) was assessed in hFOBs cultured in the osteogenic medium at 39.4°C. BMP2 (<b>C</b>), RUNX2 (<b>D</b>) and ALP (<b>E</b>)mRNA expression in hFOBs cultured in the non-differentiation medium at 39.4°C was also evaluated by real-time PCR. N = 3; mean ± SD; *<i>p</i><0.05; **<i>p</i><0.01. NC was the matched negative control: miR NC for both miR-27a mimic and siR sFRP1; miR inhibitor NC for miR-27a inhibitor; siR sFRP1+ miR-27a inhibitor NC for siR sFRP1+ miR-27a inhibitor.