Deletion of <i>m154</i> results in decreased CD48 surface density on MCMV-infected macrophages.

<p>(A) Schematic depiction of the region between the <i>m144</i> and <i>m158</i> genes in parental MCMV-GFP or wt MCMV, MCMV-GFPΔm144-m158, MCMV-GFPΔm144-m148, MCMV-GFPΔm149-m153, MCMV-GFPΔm154-m157, MCMV-GFPΔm153-m154, MCMVΔm154, and MCMVΔm154Int used in the analysis. (B) Peritoneal macrophages were mock-infected or infected for 72 h with MCMV-GFP, MCMV-GFPΔm144-m158 MCMV-GFPΔm144-m148, MCMV-GFPΔm149-m153, MCMV-GFPΔm154-m157, and MCMV-GFPΔm153-m154 at an moi of 2 and analyzed by flow cytometry for surface expression of CD48, Ly108, or CD86 as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004000#ppat-1004000-g001" target="_blank">Figure 1</a>. Open histograms represent the expression of these molecules on infected (GFP-positive) cells and shaded histograms represent the expression on uninfected (GFP-negative) cells from the same cultures. A representative experiment out of at least two performed is shown. (C) Same as in B, except that infections were performed with wt MCMV, MCMVΔm154, MCMVΔm154Int at an moi of 10 and analyzed by flow cytometry for surface expression of CD48, Ly108, and CD84. Open histograms represent the expression of these molecules on infected cultures and shaded histograms represent the expression on uninfected cultures. Isotype IgGs were used as negative controls (dashed line histograms). A representative experiment out of three performed is shown.