HTLV-1 and HTLV-2 integrate in similar genomic environments.

<p>Cells from 28 HTLV-2-infected and 16 HTLV-1-infected subjects were tested for genomic integration site preferences. (A) The ratio of the proportion of sites found in each chromosome (out of the total integration sites found for each virus) to the proportion of randomly generated (in silico) sites in the same chromosome. The yellow dashed line represents random sites (ratio = 1). (B) The number of histone marks (post-translational modifications) in three given windows across integration sites (for example, the 2k window incorporates 1,000 bases on either side of an integration site) compared to the number of histone marks in the same window across random sites. Statistical significance was assessed using the two-tailed Mann-Whitney test (* <0.05, ** <0.01, *** <0.001). (C) The odds ratio of integration within 1 kb of given ChIP-seq sites compared to random sites. The terms upstream and downstream here refer to the 5′ and 3′ sides of the integrated provirus, respectively. Statistical significance was assessed using Fisher's exact test (* <0.05, ** <0.01, *** <0.001).