Characterization of the splenocytes after rat islet transplantation into the bone marrow.

<p>The splenocytes of transplanted and naive animals were harvested seven days after transplantation. The absolute number of splenocytes was not significantly different compared to naive mice; data are shown for individual animals with the horizontal line representing the mean value (A). The percentages of CD4⁺, CD8⁺ and F4/80⁺ cells were not significantly different either. Box-and-whisker diagram are shown (B). However, splenocytes of transplanted mice showed a significantly increased level of proliferation compared to naive mice when stimulated by donor (T cell-depleted) rat irradiated splenocytes. Results are expressed in counts per minute and show the mean value of 4 naive and 6 transplanted animals (C). Abbreviation. Tx: transplanted. CPM: Count per minutes.