IHC staining for human oral cancer specimens by TMA slides.

<p>A. Levels of DNMT1 and DNMT3b were examined in cancer (CA) and non-malignant tissue (NT) by Western blotting analysis. B. Levels of DNMT1 and DNMT3b were examined in six specimens (paired cancer (CA) and adjacent non-malignant tissue (NT); by RT-PCR. The y-axis shows the ratio of target protein in cancer tissue divided by that in the non-malignant specimen. Columns, mean of three separate experiments; Bars, standard deviation (SD); *, P<0.05. C. Representative slides of IHC positive staining with DNMT1 and DNMT3b antibodies are shown by images using IPP (NT, adjacent non malignant epithelium; CA, oral cancer tissue). Images of representative slides are shown at magnifications of ×100 (upper row) and ×200 (lower row). * (+) means positive staining in cancer>adjacent NT.