ELISA of H7-HCP-Tir-Intimin–specific IgG titers of sera from immunized and non-immunized subjects.

<p>Blood was sampled from all goats at 0 d, 21 d, and 35 d (14 d post-booster) to determine IgG titers (A and B). A: Each immunized goat and naïve goat. 1–6: immunized goats; 7–12: naïve goats. The immunization induced IgG at 7 d, and this effect increased distinctly at 21 d (<i>P</i> = 0.0030) and immediately after the booster (<i>P</i> = 0.0166), but the naïve goats showed no such IgG reactions. B: Statistical analysis of average IgG titer of vaccinated goats.