Phosphorylation of specific tyrosine residues on FAK in migrating cells.

<p>Immunofluorescence images for (A, D) pY397, (B, E) pY861, and (C, F) total FAK in cells migrating on (A-C) the homogenous and (D-F) nanopatterned surfaces 3 hours after the release from the initial confinement in the 150-μm circle. (G-I) The average fluorescence intensity of 8–9 representative cells shown in A-F. (G) pY397, (H) pY861, and (I) total FAK. The error bars represent the standard deviations. The scale bars represent 20 μm. *Significant difference by a two-tailed Student's t-test (p<0.01).