Altered expression and subcellular distribution of mitochondrial fusion/fission proteins in Mn-treated C6 cells.

<p>Opa-1 and Drp-1 expression levels. Monoclonal antibodies that recognize Opa-1 or Drp-1 were used for immunoblotting analysis of total cell lysates. Reprobing with an anti-β-Actin antibody was performed to normalize for protein loading. Signals were quantified with the Image J software. Images correspond to one representative experiment (n = 3). Results are expressed as a percentage of the respective control considered as 100%. Asterisk [*] denotes Opa-1 cleavage product with a MW ∼71 kDa (<b>A</b>); Enriched-mitochondrial and cytosolic fractions were subjected to immunoblotting procedure using antibodies that recognize Opa-1 or Drp-1 proteins. Reprobing with anti-Complex III subunit core 1- OxPhos (Complex III) and anti-β-Actin antibodies was performed to normalize for loading control. Images correspond to one representative experiment (n = 2). Results are expressed as a percentage of the respective control considered as 100% (<b>B</b>). Statistically significant differences between the controls and experimental groups are indicated by: *p<0.05, **p<0.01 and ***p<0.001 vs. control. AU: Arbitrary Units.