Effect of PQ401 on proliferation of parental MHCC97H and Oxaliplatin treatment of MHCC97H-OXA and parental MHCC97H cells.

<p>(A) MHCC97H cells were incubated with varying concentrations of PQ401, beginning on 0 day. Proliferative capacity was reflected by absorbance values (O.D.) on 0, 1, 2, 3 day. Columns, mean of three experiments; bars, SD; *, proliferation significantly reduced versus controls (containing 0.1% DMSO). (B) MHCC97H, MHCC97H-OXA cells in basal medium, another MHCC97H, MHCC97H-OXA cells in medium supplemented with 10 μmol/l PQ401, were cultured for 24 hours in advance. Then, the cells of four groups were incubated with varying concentrations of Oxaliplatin for 24 h, 48 h, 72 h, 96 h, and using CCK8 assay to calculate IC₅₀ value of every group. Columns, mean of three experiments; bars, SD; **, P<0.001.