Inhibition of cell proliferation by mGluR1 antagonists.

<p>Human dermal microendothelial cells (HDEC), human umbilical vein endothelial cells (HUVEC), human lung microendothelial cells (HLEC) and the human microendothelial cell line (HMEC-1) were plated at 1×10⁵ cells/well into 96-well plates in EBM-2 basal medium (no supplements) in reduced serum (5%) containing 100 ug/ml VEGF and exposed to various mGluR1 antagonists, or vehicle (0.05% DMSO). Cell proliferation was determined on day 3 by MTT assay where formazan product was detected by measuring absorbance at 540 nm and results expressed as % control (no VEGF) where no growth was demonstrated. Both Riluzole (<b>A</b>) and BAY36-7620 (<b>B</b>) significantly inhibited cell proliferation in a dose-response manner in all EC types whereas YM 298198 (<b>C</b>) had a significant effect on cell proliferation in all the cell types except HUVEC. Results are the mean ± SEM of three experiments, performed in triplicate, where * is p<0.05 compared to their respective control cells containing VEGF.