Preprocessing of sequencing data.

<p>The barplot shows how many reads survive through each of the steps of the preprocessing pipline (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0091851#s2" target="_blank">Methods</a>). The step of going from Mapped reads to Useable reads is removal of rRNA. A large amount of reads are lost due to rRNA read removal in the RiboMinus group. The pecentage of useable reads (shown above the dotted lines) shows a steady decline with decreasing RIN. The poor performance of the RiboMinus samples can be attributed to high rRNA contamination.