Hesperidin and mangiferin did not alter the expression of A) BAX and B) BCL2 during the 12h treatment.

<p><b>Top panel:</b> Following 12h treatment of HeLa cells with hesperidin (<b>h)</b> (2.5 μg/ml, <u>4,01 μM</u>), mangiferin (5 μg/ml, <u>11.84 μM) (<b>m</b>),</u> and hesperidin (2.5<u> μ</u>g/ml, <u>4.01 μM</u>) in a presence of mangiferin (5<u> μ</u>g/ml, <u>11.84 μM</u>) (<b>h+m</b>), the mRNA levels were monitored in real - time PCR experiments. The BAX and BCL2 mRNA levels results from 2 independent experiments (n = 8) are plotted relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and 18S rRNA levels and expressed as a fold change over the EtOH control. Error bars represent standard derivations.<b>Bottom panel:</b> Following 12h of treatment of HeLa cells with hesperidin (<b>h)</b> (2.5<u> μ</u>g/ml, <u>4.01 μM</u>), mangiferin (5<u> μ</u>g/ml, <u>11.84 μM) (<b>m</b>),</u> and hesperidin (2.5<u> μ</u>g/ml, <u>4.01 μM</u>) in a presence of mangiferin (5<u> μ</u>g/ml, <u>11.84 μM</u>) (<b>h+m</b>), the protein levels of Bax and BCL2 were detected with SDS-PAGE and Western Blot and related to beta actin levels. 2 individual samples (3 μg of total protein per lane) were tested for each treatment and the experiments were repeated twice.