LTS61K affects bone marrow-derived dendritic cell (BMDC) maturation.

<p>BMDCs from BALB/c naïve mice were cultured with either <i>Dermatophagoides pteronyssinus</i> (Der p) (20 µg), LTS61K (10 µg), or lipopolysaccharide (LPS; 1 µg) for 24 hours, and then analyzed by flow cytometry. (<b>A</b>) Cell population distributions. Results for 1 representative experiment of 3 are shown. (<b>B</b>) Interleukin (IL)-6 and tumor necrosis factor (TNF)α levels. (<b>C</b>) IL-6 secretion by BMDCs was determined after pretreatment with 1 µg or 10 µg LTS61K for 4 hours and 1 µg LPS for 24 hours. N.D., not determined. NS, non-significant, Results represent the mean ± standard error of the mean for n = 6–8 mice per group. *p<0.05, **p<0.01 (Student's t-test).