Metformin treatment does not alter HFD-induced adiposity and adipose tissue inflammation.

<p>Mice were treated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0091111#pone-0091111-g001" target="_blank">Figure 1</a>. After the feeding/treatment regimen, mice were fasted for 4 hr prior to collection of tissue samples. (A) Adipose tissue histology (H&E staining). (B) Adipose tissue sections were stained for F4/80⁺ cells. (C) Adipose tissue macrophage infiltration. Percentages of mature macrophages (F4/80⁺ CD11b⁺ cells) in adipose tissue stromal cells were calculated using FACS analyses (n = 4–6). (D) Adipose tissue AMPK signaling and inflammatory signaling were examined using Western blot analyses (n = 4–6). (E) The mRNA levels of adipose genes were quantified using real-time PCR (n = 4–6). For bar graphs (C and E), data are means ± SE.