cMET but not EGFR inhibitors reduced cell migration in HGF stimulated lung tumor spheroids.

<p>A) Day 4 lung spheroids generated from H292, H1650, H1975 and A549 were treated with Erlotinib, Cetuximab, Crizotinib and MetMab in a dilution series in the presence of 20 ng/ml of HGF for 48 hours to stimulate cell migration. Total area (μm²) of migrating and spheroid were determined by using bright field images in a fully automated Operetta high content imaging system (Perkin Elmer). Cell migration (total area) was normalized to media only control to create a percentage cell migration to control. Data are means <u>+</u> SEM with two to five replicates and is representative of two independent experiments. B) Representative bright field images showing drug response after 48 hours in 3D spheroids in cell migration at the highest concentration. Magnification: 2x objective, scan bar 1 mm.