Neonatal touch dome Merkel cells are innervated by Ret⁺ and NFH⁺ fibers.

<p>A-B, Triple staining of the back hairy skin of P0 <i>Ret^{CFP +/}</i>⁻ mice with antibodies against CK8, GFP and NFH. Two representative examples of touch domes are shown in A and B. CFP⁺ Merkel cell innervating fibers are indicated by red arrows, NFH⁺ fibers are indicated by green arrows, and the overlap spots of Ret⁺ and NFH⁺ fibers are indicated by yellow arrows. C, Quantification of the percentages of Merkel cells innervated by Ret⁺ and NFH⁺ fibers out of total Merkel cells in the touch domes. D, Quantification of the percentages of different fiber innervation out of the innervated Merkel cells in the touch domes. Scale bar, 20 μm. n = 3 animals. See also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0092027#pone-0092027-t001" target="_blank">Table 1</a>.