Specificity characterization of antibody generated against RBC ghosts and <i>Listeria monocytogenes</i> in rabbit sera.

<p><b>A</b>) ELISA analysis: Antibody titer against <i>Listeria</i> surface antigens and RBC ghost proteins. Data are represented as mean ± standard error of three independent experiments (<i>P</i> values; Anti-RBC Ab vs control <0.01, Anti-List Ab vs control <0.01). <b>B</b>) Fluorescence microscopy: Anti-<i>Listeria</i> antibody interaction with cell surface proteins of <i>Listeria monocytogenes</i> (<b>a and b</b>), anti-RBC antibody binding to human RBCs (<b>c and d</b>). <b>C</b>) Dot blot analysis: <b>a</b> and <b>b</b> exhibit recognition of <i>Listeria</i> surface antigens by monospecific anti-<i>Listeria</i> antibody and interaction of RBC ghost proteins by anti-RBC antibody respectively. The controls where InlB antigen of <i>Listeria</i> (c) and RBC antigens (d) were spotted onto PVDF membrane respectively. InlB antigens were allowed to react with anti-RBC while RBC antigens were allowed to interact with anti-InlB monospeific antibodies respectively. At least three independent experiments were performed for each sample and data are representative of three independent experiments with similar observations.