The ROS level and oxidative stress in injured kidney tissues in early stage.

<p>(A) DCFH-DA analysis showed relative ROS level in kidney tissues at different time points after reperfusion (n = 6).*<i>P</i><0.05, MVs versus VEHICLE; #<i>P</i><0.05, SHAM versus VEHICLE. The ROS level in renal tissues of SHAM-rats was used for calibration (dotted line). (B) Quantification of MDA concentration in renal tissues at different time points after reperfusion (n = 6).*<i>P</i><0.05, SHAM versus VEHICLE; #<i>P</i><0.05, MVs versus VEHICLE. (C) Representative micrographs of 8-OHdg immunostaining in SHAM operation, MVs and VEHICLE rats at 48h showed higher staining in the VEHICLE group but reduced by MVs. (magnification ×200, Scale bar = 50 μm). (D) Oxyblot analysis showed that there was less protein-carbonylation in MVs group compared with VEHICLE group at 24h and 48h after reperfusion. (E) Digital quantification of gray value of oxyblot bands showed the level of injured protein by ROS in each group (n = 3). *<i>P</i><0.05, MVs versus VEHICLE; #<i>P</i><0.05, SHAM versus VEHICLE. The gray value of SHAM group was used for calibration (dotted line).