SOCS3 gene silencing in lung.

<p><b>A</b>. Red-labeled siRNA localization in mouse lung after intranasal delivery determined by confocal microscopy. 2 μM of siRNA was administered. 1: control mouse lung after 48 h after administration of distilled water. 2 and 4: Lung airway images after 24 h from siRNA delivery. 3 and 5: Lung airways 48 h after siRNA administration. <b>B</b>. Relative SOCS3 mRNA levels were determined by real-time quantitative PCR. The results show relative gene expressions as determined by the ΔΔC_T method. <b>C</b>. SOCS3 Western blot analysis from lungs was achieved using an antibody against SOCS3 and β-actin. Lane 1: lung lysate from the SS group; lane 2: lung lysate from the OVA group; Lane 3: lung lysate from the OVA+siNT group; and lane 4: lung lysate from the OVA+siSOCS3 group. β-actin was used as a loading control. The picture is a representative example of several mice, all displaying similar results. <b>D</b>. SOCS3 bands were quantified by densitometry. SS group (mean ±SD, n = 13); OVA group (mean ±SD, n = 12); OVA siNT-treated group (mean ±SD, n = 12); OVA siSOCS3-treated group (mean ±SD, n = 15). ‡<i>P</i><0.001 and *<i>P</i><0.05 between groups.