New cytotoxic spirostane saponin and biflavonoid glycoside from the leaves of <i>Acacia saligna</i> (Labill.) H.L. Wendl.

<div><p>New naturally occurring spirostane saponin (25<i>S</i>)-5β-spirostan-3β-yl-3-<i>O</i>-β-d-xylopyranosyl(1 → 3)-<i>O</i>-β-d-xylopyranosyl(1 → 4)-β-d-galactopyranoside (<b>6</b>) and biflavonoid glycoside myricetin-3-<i>O</i>-rhamnoside (C7-<i>O</i>-C7) myricetin-3-<i>O</i>-rhamnoside (<b>4</b>) along with a series of known compounds erythrodiol (<b>1</b>), 3β-<i>O</i>-<i>trans</i>-<i>p</i>-coumaroyl-erythrodiol (<b>2</b>), quercetin-3-<i>O</i>-α-l-rhamnoside (<b>3</b>) and myricetin-3-<i>O</i>-α-l-rhamnoside (<b>5</b>) were separated from the leaves of <i>Acacia saligna</i> (Labill.), H.L. Wendl. Compounds <b>1</b> and <b>2</b> were separated for the first time from genus <i>Acacia.</i> The structures of compounds <b>1</b>–<b>6</b> were established on the basis of extensive 1D and 2D NMR spectroscopic techniques in combination with EI-MS and HR-ESI-MS. These compounds were screened for their antioxidant and cytotoxic activities against HEPG2 (liver cancer) cell line and significant results were obtained.</p>