IDUA and anti-IDUA antibody production after MSC-KO-IDUA transplantation.

<p>For the first transplantation (A), four million MSC-KO cells were nucleofected with pCMV-SB11 and pT2-CAGGS-IDUA and transplanted into the peritoneum. Three mice died during this experiment: 88.1.2, 88.1.5 and 89.1.3. Approximately, 40 days after the first cell transplantation, the second transplantation was performed using MSC-KO that were nucleofected with pCMV-SB100X and pT2-CAGGS-IDUA (B). Here, two new IDUA-KO mice were included (#). A month after the second transplantation, a third transplantation was performed using the same procedure as the second (C). Fifteen days after the last cell transplantation, blood samples were collected to quantify the anti-IDUA antibody (D). *p<0.05 when comparing the IDUA-KO group to other groups. A two-way ANOVA with the Bonferroni post hoc test was used. HT: heterozygous mouse. KO: IDUA-KO mouse.