Nitrite inhibits thrombin-induced platelet activation in the presence of deoxygenated erythrocytes.

<p>Representative flow diagrams demonstrate the inhibitory effects of nitrite on the thrombin-induced activation of platelets. Platelet activation was analyzed by monitoring the percentage of cells expressing P-selectin (A, B, C) and activated gpIIb/IIIa (D, E, F) after thrombin (1 U/mL) stimulation in both control (blue) and nitrite (1 μM; orange) treated group in the presence of erythrocytes (Hct 42.6%) with different oxygen concentrations [pO₂; 97.4±4.2 mmHg (A,D), 58.2±6.2 mmHg (B,E) and 26.7±2.8 mmHg (C,F), n = 6].