DENV serotypes, viremia titer, and anti-DENV antibody isotypes in DENV-infected patients.

a<p>ID, patient identification.</p>b<p>Dengue disease was determined by examining the clinical symptoms of patients according to the WHO criteria.</p>c<p>DENV serotype was determined by RT-PCR with universal and serotype-specific primers <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0092173#pone.0092173-Yenchitsomanus1" target="_blank">[31]</a>.</p>d<p>Viremia titers were determined in a focus-forming immunoassay in semi-adherent K562 cells <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0092173#pone.0092173-Kurosu1" target="_blank">[30]</a>.</p>e<p>IgM in patient serum was detected by Dengue Virus IgM Capture DxSelect. An index value of >1.00 was interpreted as positive (POS) and an index value of <1.00 was interpreted as negative (NEG).</p>f<p>IgG in patient serum was detected by Dengue Virus IgG DxSelect. An index value of >1.00 was interpreted as positive (POS) and an index value of <1.00 was interpreted as negative (NEG).</p>g<p>Cases with an IgM/IgG index ratio of ≤1.2 were diagnosed as secondary infections <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0092173#pone.0092173-Shu1" target="_blank">[32]</a>.</p>h<p>Patient’s blood specimen was used for huMAb preparation as described elsewhere <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0092173#pone.0092173-Guy1" target="_blank">[24]</a>. ND, not detectable.