<p>Positive expression of ALB, AFP, HNF-4α and CK18 were observed in patient sera induction as well as hepatogenic cocktail induction group, compared to untreated hMSCs and normal sera induction group. No differences in expressions were observed between the two positive induction groups, but it was significantly lesser compared to HepG2 (*<i>p</i><0.05). The expressions of genes were normalized to GAPDH and mRNA of untreated hMSCs was used as calibrator.</p
<p>A, Expression of PPARγ, FAS, CPT 1, ACO in the liver tissue; B Expression of IL-1β, IL-6, TNFα in...
Abstract Background Housekeeping genes are routinely used as endogenous references to account for ex...
<p>Gene expression was determined by real-time qPCR in n = 14 patients. Char...
<p>Hepatocyte-specific gene expression analyzed by qRT-PCR in hUC-MSCs and hepatic differentiated hU...
<p><b>A.</b> RT-PCR analysis of liver-specific gene expression. RNA samples were extracted from undi...
<p>Expression of genes encoding nuclear receptors (A), phase I enzymes (B), phase II enzymes (C), an...
<p>(A) Analysis of expression of the pluripotency markers OCT4 and REX1 by qRT-PCR in: undifferentia...
<p>(Ai) Albumin ELISA of the culture media collected from various hepatic induction groups and contr...
<div><p>Reference genes (RG) as sample internal controls for gene transcript level analyses by quant...
<p>(A) QRT-PCR analysis of mRNA form human hepatic stellate cell line (LX-2) and human hepatocellula...
<p>(<b>A</b>) c-Met mRNA. (<b>B</b>) Hepatocyte growth factor mRNA. mRNA levels for c-Met, hepatocyt...
<p>(<b>A</b>) Differentiation scheme of hESC towards Hepatocyte-like cells (Hep-like). (<b>B</b>) He...
<p>(A) mRNA expression of the key genes for inflammatory response and acute phase responses. (B) mRN...
<p>(<b>A</b>) Gene expression analysis using real-time PCR. Gene expression of HNF4α, Cyp3A, Cyp1A2,...
<p>Self-renewing hiHSCs, clones AFB1-1 and NGC1-1, differentiate into hepatocyte-like cells with the...
<p>A, Expression of PPARγ, FAS, CPT 1, ACO in the liver tissue; B Expression of IL-1β, IL-6, TNFα in...
Abstract Background Housekeeping genes are routinely used as endogenous references to account for ex...
<p>Gene expression was determined by real-time qPCR in n = 14 patients. Char...
<p>Hepatocyte-specific gene expression analyzed by qRT-PCR in hUC-MSCs and hepatic differentiated hU...
<p><b>A.</b> RT-PCR analysis of liver-specific gene expression. RNA samples were extracted from undi...
<p>Expression of genes encoding nuclear receptors (A), phase I enzymes (B), phase II enzymes (C), an...
<p>(A) Analysis of expression of the pluripotency markers OCT4 and REX1 by qRT-PCR in: undifferentia...
<p>(Ai) Albumin ELISA of the culture media collected from various hepatic induction groups and contr...
<div><p>Reference genes (RG) as sample internal controls for gene transcript level analyses by quant...
<p>(A) QRT-PCR analysis of mRNA form human hepatic stellate cell line (LX-2) and human hepatocellula...
<p>(<b>A</b>) c-Met mRNA. (<b>B</b>) Hepatocyte growth factor mRNA. mRNA levels for c-Met, hepatocyt...
<p>(<b>A</b>) Differentiation scheme of hESC towards Hepatocyte-like cells (Hep-like). (<b>B</b>) He...
<p>(A) mRNA expression of the key genes for inflammatory response and acute phase responses. (B) mRN...
<p>(<b>A</b>) Gene expression analysis using real-time PCR. Gene expression of HNF4α, Cyp3A, Cyp1A2,...
<p>Self-renewing hiHSCs, clones AFB1-1 and NGC1-1, differentiate into hepatocyte-like cells with the...
<p>A, Expression of PPARγ, FAS, CPT 1, ACO in the liver tissue; B Expression of IL-1β, IL-6, TNFα in...
Abstract Background Housekeeping genes are routinely used as endogenous references to account for ex...
<p>Gene expression was determined by real-time qPCR in n = 14 patients. Char...